CST, 3658T, AceCS1 (D19C6) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying acetyl-CoA synthetase. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, AceCS1 (D19C6) Rabbit Monoclonal Antibody (CST #3658) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity AceCS1 (D19C6) Rabbit Monoclonal Antibody detects endogenous levels of total cytoplasmic acetyl-CoA synthetase (AceCS1). Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu49 of human cytoplasmic acetyl-CoA synthetase (AceCS1) protein. Background Cytoplasmic acetyl-CoA synthetase (AceCS1) catalyzes the conversion of acetate and CoA to acetyl-CoA (1, 2). Acetyl-CoA synthesized by AceCS1 is used for fatty acid and lipid biosynthesis (1, 2). Studies suggest that this enzyme is regulated by sterol regulatory element-binding proteins (2). It was also shown that this enzyme is acetylated by protein acetyltransferases in cells (3, 4). SIRT1 deacetylates this enzyme at Lys661 resulting in its activation and the increase of fatty acid synthesis (3, 4). Alternate Names ACAS2; AceCS; AceCS1; acetate thiokinase; Acetate--CoA ligase; acetate-CoA ligase; Acetyl-CoA synthetase; Acetyl-CoA synthetase 1; acetyl-Coenzyme A synthetase 2 (ADP forming); Acetyl-coenzyme A synthetase, cytoplasmic; ACS; ACSA; ACSS2; Acyl-activating enzyme; acyl-CoA synthetase short chain family member 2; Acyl-CoA synthetase short-chain family member 2; cytoplasmic acetyl-coenzyme A synthetase; dJ1161H23.1; DKFZp762G026; Propionate--CoA ligase Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 78 Source/Isotype: Rabbit IgG