CST, 3661S, Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody

Polyclonal Antibody for studying acetyl-CoA carboxylase 1 (Ser80) phosphate/acetyl-CoA carboxylase 2 (Ser222) phosphate. Validated for Western Blotting,Immunoprecipitation,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody (CST #3661) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin) Specificity / Sensitivity Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody detects endogenous levels of ACC only when phosphorylated at serine 79. This antibody recognizes both ACCalpha and ACCbeta. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser79 of rat ACC. Antibodies are purified by protein A and peptide affinity chromatography. Background Acetyl-CoA carboxylase (ACC) catalyzes the carboxylation of acetyl-CoA to malonyl-CoA (1). It is the key enzyme in the biosynthesis and oxidation of fatty acids (1). In rodents, the 265 kDa ACC1 (ACCα) form is primarily expressed in lipogenic tissues, while 280 kDa ACC2 (ACCβ) is the main isoform in oxidative tissues (1,2). However, in humans, ACC2 is the predominant isoform in both lipogenic and oxidative tissues (1,2). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (3). ACC is a potential target of anti-obesity drugs (4,5). Alternate Names ACAC; ACACA; ACACAD; ACACB; ACC; ACC-alpha; ACC-beta; ACC1; ACC2; ACCA; ACCB; Acetyl-CoA carboxylase 1; Acetyl-CoA carboxylase 2; acetyl-CoA carboxylase alpha; acetyl-CoA carboxylase beta; acetyl-CoA carboxylase-alpha; acetyl-Coenzyme A carboxylase alpha; acetyl-Coenzyme A carboxylase beta; Biotin carboxylase; HACC275 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 280 SOURCE: Rabbit