CST, 3664S, RhoE (4) Mouse Monoclonal Antibody

Monoclonal Antibody for studying RhoE. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, RhoE (4) Mouse Monoclonal Antibody (CST #3664) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity RhoE (4) Mouse Monoclonal Antibody detects endogenous levels of total RhoE protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with full length recombinant human RhoE. Background Rho family small GTPases act as molecular switches that regulate processes such as cell migration, adhesion, proliferation and differentiation. Typically, they are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of bound GDP for GTP, and are inhibited by GTPase activating proteins (GAPs), which catalyze the hydrolysis of GTP to GDP (1). Rnd1, Rnd2 and RhoE/Rnd3 comprise the evolutionarily divergent Rnd family of Rho-type small GTPases, which lack GTPase activity and therefore remain in a GTP-bound state (2, reviewed in 3). RhoE/Rnd3 activity leads to a decrease in stress fibers and increased cell migration, at least in part through regulation of the Rho-dependent kinase ROCK1 (4). Activity of RhoE/Rnd3 itself is regulated by ROCK1, which phosphorylates RhoE/Rnd3 at Ser11, enhancing its activity (5). RhoE/Rnd3 has been implicated in inhibition of DNA-damage induced apoptosis (6) and cell cycle arrest (7). In keratinocytes, RhoE/Rnd3 regulates differentiation through its effects on both proliferation and adhesion (8). Alternate Names ARHE; memB; Protein MemB; ras homolog gene family, member E; Rho family GTPase 3; Rho-related GTP-binding protein Rho8; Rho-related GTP-binding protein RhoE; RHO8; RHOE; RND3; small GTP binding protein Rho8 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 29 Source/Isotype: Mouse IgG1