CST, 3669T, p115 RhoGEF (D25D2) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ARHGEF1. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, p115 RhoGEF (D25D2) Rabbit Monoclonal Antibody (CST #3669) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity p115 RhoGEF (D25D2) Rabbit Monoclonal Antibody detects endogenous levels of total p115 RhoGEF protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human p115 RhoGEF. Background The Rho family small GTPases, including Rho, Rac and cdc42, act as molecular switches, regulating processes such as cell migration, adhesion, proliferation and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of bound GDP for GTP, and inhibited by GTPase activating proteins (GAPs), which catalyze the hydrolysis of GTP to GDP. A third level of regulation is provided by the stoichiometric binding of Rho GDP dissociation inhibitor (RhoGDI) (1). G-protein coupled receptors (GPCRs) at the cell surface signal through heteromeric G proteins to small GTPases such as Rho, which then signal to downstream effector molecules (2). p115 RhoGEF/ArhGEF1 and its family members PDZ-RhoGEF (PRG), and LARG are stimulated by heteromeric G proteins and thus couple signaling from GPCRs to Rho small GTPases (3-6). In a mouse model of asthma, p115 RhoGEF is necessary for T cells to enable airway inflammation and hyperreactivity (7). Alternate Names 115 kDa guanine nucleotide exchange factor; 115-kD protein; ARHG1; ARHGEF1; GEF1; IMD62; LBCL2; LSC; Lsc homolog; p115-RhoGEF; p115RhoGEF; Rho guanine nucleotide exchange factor (GEF) 1; Rho guanine nucleotide exchange factor 1; Sub1.5 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 115 Source/Isotype: Rabbit