CST, 3673S, Cdc45 Antibody

Polyclonal Antibody for studying CDC45L. Validated for Western Blotting. Highly specific and rigorously validated in-house, Cdc45 Antibody (CST #3673) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Cdc45 Antibody recognizes endogenous levels of total cdc45 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human cdc45 protein. Antibodies are purified using protein A and peptide affinity chromatography. Background The initiation of DNA replication in mammalian cells is a highly coordinated process that ensures duplication of the genome only once per cell division cycle. Origins of replication are dispersed throughout the genome and their activities are regulated via the sequential binding of pre-replication and replication factors. The origin recognition complex (ORC) is thought to bind to chromatin throughout the cell cycle (1,2). The pre-replication complex (Pre-RC) forms in late mitosis/early G1 phase with the binding of CDT1 and cdc6 to the origin, which allows binding of the heterohexameric MCM2-7 complex. The MCM complex is thought to be the replicative helicase and formation of the Pre-RC is referred to as chromatin licensing. Subsequent initiation of DNA replication requires the activation of the S-phase promoting kinases cdk2 and cdc7. Cdc7 phosphorylates MCM proteins bound to chromatin and, in conjunction with CDT1, recruits the replication factor cdc45 (3-5). Cdc45 is required for formation of pre-initiation complexes at the G1/S transition and for activation of replication origins. The level of cdc45 protein expression is associated with the proliferative status of the cell or tissue. Terminally differentiated and senescent cells lack cdc45 and highly proliferative cell lines express high levels of cdc45 (6). Alternate Names CC45L; Cdc45; CDC45 (cell division cycle 45, S.cerevisiae, homolog)-like; CDC45 cell division cycle 45 homolog; CDC45 cell division cycle 45 homolog (S. cerevisiae); CDC45 cell division cycle 45-like; CDC45-related protein; CDC45L; CDC45L2; Cell division control protein 45 homolog; cell division cycle 45; cell division cycle 45 homolog; cell division cycle 45-like 2; human CDC45; MGORS7; PORC-PI-1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 65 SOURCE: Rabbit