CST, 3675S, Phospho-Myosin Light Chain 2 (Ser19) Mouse Monoclonal Antibody

Monoclonal Antibody for studying Myosin Light Chain 2 (Ser20) phosphate. Validated for Western Blotting,Simple Western™,Immunofluorescence (Immunocytochemistry). Available in 3 sizes. Highly specific and rigorously validated in-house, Phospho-Myosin Light Chain 2 (Ser19) Mouse Monoclonal Antibody (CST #3675) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunofluorescence (Immunocytochemistry): 1:200 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 95377 . Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Phospho-Myosin Light Chain 2 (Ser19) Mouse Monoclonal Antibody detects endogenous levels of myosin light chain 2 (smooth muscle) only when phosphorylated at serine 19. This antibody does not cross-react with the cardiac isoform of myosin light chain 2. Species Reactivity: Human, Mouse, Rat, Bovine, Pig Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser19 of human myosine light chain 2 (smooth muscle). Background Myosin is composed of six polypeptide chains: two identical heavy chains and two pairs of light chains. Myosin light chain 2 (MLC2), also known as myosin regulatory light chain (MRLC), RLC, or LC20, has many isoforms depending on its distribution. In smooth muscle, MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca /calmodulin-dependent manner (1). This phosphorylation is correlated with myosin ATPase activity and smooth muscle contraction (2). ROCK also phosphorylates Ser19 of smooth muscle MLC2, which regulates the assembly of stress fibers (3). Phosphorylation of smooth muscle MLC2 at Ser1/Ser2 and Ser9 by PKC and cdc2 has been reported to inhibit myosin ATPase activity (4,5). Phosphorylation by cdc2 controls the timing of cytokinesis (5). Transgenic mice lacking phosphorylation sites on the cardiac muscle isoform show morphological and functional abnormalities (6). Alternate Names 20 kDa myosin light chain; epididymis secretory sperm binding protein; LC20; MGC3505; MLC-2C; MLC2; MRLC1; MYL9; myosin light chain 9; myosin regulatory light chain 1; Myosin regulatory light chain 2, smooth muscle isoform; Myosin regulatory light chain 9; Myosin regulatory light chain MRLC1; Myosin regulatory light polypeptide 9; Myosin RLC; myosin, light chain 9, regulatory; myosin, light polypeptide 9, regulatory; MYRL2 Specification REACTIVITY: H M R B Pg SENSITIVITY: Endogenous MW (kDa): 18 Source/Isotype: Mouse IgG1