CST, 3676T, Acetyl-CoA Carboxylase (C83B10) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying acetyl-CoA carboxylase 1/acetyl-CoA carboxylase 2. Validated for WB,WB,IP,IHC,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Acetyl-CoA Carboxylase (C83B10) Rabbit Monoclonal Antibody (CST #3676) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:50 - 1:200 Immunofluorescence (Immunocytochemistry): 1:100 - 1:200 Flow Cytometry (Fixed/Permeabilized): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 52923 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Acetyl-CoA Carboxylase (C83B10) Rabbit Monoclonal Antibody detects endogenous levels of all isoforms of acetyl-CoA carboxylase protein. Species Reactivity: Human, Mouse, Rat, Hamster Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser523 of human acetyl-CoA carboxylase α1. Background Acetyl-CoA carboxylase (ACC) catalyzes the carboxylation of acetyl-CoA to malonyl-CoA (1). It is the key enzyme in the biosynthesis and oxidation of fatty acids (1). In rodents, the 265 kDa ACC1 (ACCα) form is primarily expressed in lipogenic tissues, while 280 kDa ACC2 (ACCβ) is the main isoform in oxidative tissues (1,2). However, in humans, ACC2 is the predominant isoform in both lipogenic and oxidative tissues (1,2). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (3). ACC is a potential target of anti-obesity drugs (4,5). Alternate Names ACAC; ACACA; ACACAD; ACACB; ACC; ACC-alpha; ACC-beta; ACC1; ACC2; ACCA; ACCB; Acetyl-CoA carboxylase 1; Acetyl-CoA carboxylase 2; acetyl-CoA carboxylase alpha; acetyl-CoA carboxylase beta; acetyl-CoA carboxylase-alpha; acetyl-Coenzyme A carboxylase alpha; acetyl-Coenzyme A carboxylase beta; Biotin carboxylase; HACC275 Specification REACTIVITY: H M R Hm SENSITIVITY: Endogenous MW (kDa): 280 Source/Isotype: Rabbit IgG