CST, 36788S, GCH1 (F8T4M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying GCH1. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, GCH1 (F8T4M) Rabbit Monoclonal Antibody (CST #36788) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:1600 - 1:3200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity GCH1 (F8T4M) Rabbit Monoclonal Antibody recognizes endogenous levels of total GCH1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly52 of human GCH1 protein. Background GTP cyclohydrolase 1 (GCH1) catalyzes the rate-limiting step of tetrahydrobiopterin (BH ) biosynthesis. Studies show that BH functions as a radical-trapping antioxidant and protects cells against ferroptosis independently of GPX4. In addition, dihydrofolate reductase (DHFR) is required to maintain BH levels by regenerating the compound (1,2). Furthermore, deficiency of spliceosomal GTPase Eftud2 in mouse cerebellar Purkinje cells (PCs) causes severe ferroptosis, PC degeneration, dyskinesia, and cerebellar atrophy. Ablation of in PCs downregulates expression through mis-splicing of , activating ferroptosis (3). Alternate Names dystonia 14; DYT14; DYT5; DYT5a; GCH; GCH1; GTP cyclohydrolase 1; GTP cyclohydrolase I; GTP-CH-1; GTP-CH-I; GTPCH1; guanosine 5'-triphosphate cyclohydrolase I; HPABH4B Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 28 Source/Isotype: Rabbit IgG