CST, 36804S, AK-7

Chemical Modulators for studying in the research area. Product Usage Information AK-7 is supplied as a lyophilized powder. For a 10 mM stock, reconstitute 5 mg of powder in 1.14 mL of DMSO. Working concentrations and length of treatment can vary depending on the desired effect. Storage Store lyophilized at room temperature, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles . Background The brain-permeable small-molecule AK-7 inhibits the NAD-dependent deacetylase sirtuin 2 (SirT2) which is involved in cytoskeletal regulation and progression through mitosis. The inhibitor AK-7 specifically binds and inhibits SirT2 (IC = 15.5 mM) and has no apparent effect on SirT1 or SirT3. AK-7 reduced total cholesterol in primary striatal neurons and reduced cholesterol in cultured naïve neuronal cells and brain slices from wild-type mice (1). Treatment with AK-7 improved motor function, extended survival, and reduced brain atrophy in a pair of genetic mouse models of Huntington's disease (2). In a study of aging in a rat model of Parkinson's disease, AK-7 inhibition revealed a potential role for SirT2 in Parkinson's neurodegeneration, and suggested that AK-7 may have a beneficial neuroprotective effect (3). Similar results were seen in a mouse model of Parkinson's disease, but AK-7 was not able to counteract neurodegenerative processes associated with amyotrophic lateral sclerosis (ALS) or cerebral ischemia (4).