CST, 36809S, Caspase-14 (D3J3D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Casp14. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Caspase-14 (D3J3D) Rabbit Monoclonal Antibody (CST #36809) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Caspase-14 (D3J3D) Rabbit Monoclonal Antibody recognizes endogenous levels of total caspase-14 protein. Endogenous levels of the p20 activated subunit have not been detected. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala71 of human caspase-14 protein. Background Caspases are a family of cysteine proteases that play an essential role in carrying out apoptosis. Caspase-14, also named MICE, is a unique member of the caspase family with restricted expression; it is found in embryonic tissues and adult skin (1,2). Caspase-14 is weakly processed into p18 and p11 subunits by caspase-8 (2). Caspase-14 may not play a role in apoptosis (2), but instead may regulate keratinocyte differentiation (3). Expression of caspase-14 may protect from psoriasis (4,5) and irradiation damage (6). Caspase-14 may also be responsible for proteolytic processing of filaggrin during terminal differentiation of keratinocytes (6). Alternate Names apoptosis-related cysteine protease; ARCI12; CASP-14; CASP14; caspase 14; caspase 14, apoptosis-related cysteine peptidase; caspase 14, apoptosis-related cysteine protease; Caspase-14; Caspase-14 subunit p10; Caspase-14 subunit p10, mature form; Caspase-14 subunit p17, mature form; Caspase-14 subunit p19; Caspase-14 subunit p20, intermediate form; Caspase-14 subunit p8, intermediate form; CASPE; MGC119078; MGC119079 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 28-30 Source/Isotype: Rabbit IgG