CST, 3683S, GAPDH (14C10) Rabbit Monoclonal Antibody (HRP Conjugate)

Monoclonal Antibody for studying GAPDH. Validated for Western Blotting. Highly specific and rigorously validated in-house, GAPDH (14C10) Rabbit Monoclonal Antibody (HRP Conjugate) (CST #3683) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 140 mM NaCl, 3 mM KCI, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity GAPDH (14C10) Rabbit Monoclonal Antibody (HRP Conjugate) detects endogenous levels of total GAPDH protein. Species Reactivity: Human, Mouse, Rat, Monkey, Bovine, Pig Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide near the carboxy terminus of human GAPDH. The antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. Background Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue (1), GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis (2), gene expression (3), and nuclear transport (4). GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases (4,5). Alternate Names aging-associated gene 9 protein; epididymis secretory sperm binding protein Li 162eP; G3P; G3PD; GAPD; GAPDH; glyceraldehyde 3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase; HEL-S-162eP; MGC88685; OCAS, p38 component; Oct1 coactivator in S phase, 38 Kd component; Peptidyl-cysteine S-nitrosylase GAPDH Specification REACTIVITY: H M R Mk B Pg SENSITIVITY: Endogenous MW (kDa): 37 Source/Isotype: Rabbit IgG