CST, 36866S, Phospho-Parkin (Ser65) Antibody

Polyclonal Antibody for studying Parkin (Ser65) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-Parkin (Ser65) Antibody (CST #36866) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-Parkin (Ser65) Antibody recognizes transfected levels of Parkin protein only when phosphorylated at Ser65. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser65 of human Parkin protein. Antibodies are purified by peptide affinity chromatography. Background Parkin is a protein of 465 amino acids with an amino-terminal ubiquitin domain and a carboxy-terminal RING-box (1). In the case of autosomal recessive juvenile Parkinsonism (AR-JP), deletions have been found in the gene on chromosome 6 encoding the protein Parkin (2). Parkin is phosphorylated by PINK1 at site Ser65. Phosphorylation at this site has been shown to be necessary for Parkin activation (3). Alternate Names AR-JP; E3 ubiquitin-protein ligase parkin; LPRS2; PARK2; parkin; parkin 2; parkin RBR E3 ubiquitin protein ligase; Parkin RBR E3 ubiquitin-protein ligase; Parkinson disease (autosomal recessive, juvenile) 2, parkin; Parkinson disease protein 2; Parkinson juvenile disease protein 2; parkinson protein 2 E3 ubiquitin protein ligase; parkinson protein 2, E3 ubiquitin protein ligase (parkin); PDJ; PRKN; PRKN2 Specification REACTIVITY: H SENSITIVITY: Transfected Only MW (kDa): 50 SOURCE: Rabbit