CST, 3686T, p27 Kip1 (D69C12) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying p27 Kip1. Validated for WB,IP,IF,F. Available in 3 sizes. Highly specific and rigorously validated in-house, p27 Kip1 (D69C12) Rabbit Monoclonal Antibody (CST #3686) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600 Flow Cytometry (Fixed/Permeabilized): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 34979 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity p27 Kip1 (D69C12) Rabbit Monoclonal Antibody detects endogenous levels of total p27 Kip1 protein. Species Reactivity: Human, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg19 of human p27 Kip1 protein. Background p27 Kip1 is a member of the Cip/Kip family of cyclin-dependent kinase inhibitors. Like its relatives, p57 Kip2 and p21 Waf1/Cip1, the ability to enforce the G1 restriction point is derived from its inhibitory binding to CDK2/cyclin E and other CDK/cyclin complexes. Expression levels of p27 are upregulated in quiescent cells and in cells treated with cAMP or other negative cell cycle regulators. Downregulation of p27 can be induced by treatment with interleukin-2 or other mitogens; this involves phosphorylation of p27 and its degradation by the ubiquitin-proteasome pathway (1-4). Alternate Names CDKN1B; CDKN4; CDN1B; cyclin dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor 1B; cyclin-dependent kinase inhibitor 1B (p27, Kip1); Cyclin-dependent kinase inhibitor p27; KIP1; MEN1B; MEN4; p27Kip1 Specification REACTIVITY: H R Mk SENSITIVITY: Endogenous MW (kDa): 27 Source/Isotype: Rabbit IgG