CST, 3687S, ASM Antibody

Polyclonal Antibody for studying SMPD1. Validated for Western Blotting. Highly specific and rigorously validated in-house, ASM Antibody (CST #3687) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity ASM Antibody detects endogenous levels of total human ASM protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues at the carboxyl terminus of human ASM. Antibody was purified by protein A and peptide affinity chromatography. Background Sphingomyelinases (SMases) catalyze sphingomyelin hydrolysis to produce ceramide and phosphocholine (1). Ceramide is an important bioactive lipid triggering signal transduction in cell proliferation, apoptosis, and differentiation (1,2). Many SMases have been described and categorized based on their optimum pH activity, cation dependence, tissue distribution, and subcellular localization (1). These include a lysosomal acid SMase, a Zn -dependent secreted acid SMase, a membrane-bound Mg -dependent neutral SMase, a Mg -independent neutral SMase, and an alkaline SMase. Acid sphingomyelinase (ASM or SMPD1) is a lysosomal enzyme responsible for the hydrolysis of sphingomyelin to ceramide and phosphocholine. The ASM gene encodes three proteins, ASM-1, ASM-2, and ASM-3, of which ASM-1 is the only catalytically active enzyme (3,4). ASM-1 can exist as a 70 kDa form as well as a 57 kDa proteoytic product (5). Expression of ASM is induced during monocytic cell differentiation (6). Defects in the ASM gene are associated with type A and type B Niemann-Pick disease (7). Alternate Names Acid sphingomyelinase; ASM; aSMase; Niemann-Pick type A/B; NPD; SMPD1; Sphingomyelin phosphodiesterase; sphingomyelin phosphodiesterase 1; sphingomyelin phosphodiesterase 1, acid lysosomal Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 57, 70 SOURCE: Rabbit