CST, 36966T, GATA-4 (D3A3M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying GATA4. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, GATA-4 (D3A3M) Rabbit Monoclonal Antibody (CST #36966) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:400 Flow Cytometry (Fixed/Permeabilized): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 46576 . Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity GATA-4 (D3A3M) Rabbit Monoclonal Antibody recognizes endogenous levels of total GATA-4 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human GATA-4 protein. Background GATA proteins comprise a group of transcription factors that are related by the presence of conserved zinc finger DNA binding domains, which bind directly to the nucleotide sequence core element GATA (1-3). There are six vertebrate GATA proteins, designated GATA-1 to GATA-6 (3). GATA-4 is crucial for cardiomyocyte differentiation (4), and not surprisingly, mutations in the gene are implicated in many cardiac diseases, such as tetralogy of Fallot (5), familial and sporadic dilated cardiomyopathy (6,7), and atrial septal defect (8). GATA-4 and GATA-6 together maintain intestinal epithelial structure by regulating enterocyte gene expression (9). They also have overlapping roles in steroidogenesis and genital ridge formation during gonadal development (10). GATA-4 is also a required factor for bone mineralization in osteoblast progenitor cells, where it suppresses TGF-β and activates BMP signaling (11). Research studies have uncovered crosstalk among the transcription factors GATA-4, GATA-6, and KLF5 that promotes gastric cancer development (12). Additional studies describe the role of histone H3K9 hyperacetylation on the GATA-4 promoter in fetal mice that have been exposed to ethanol (13). Alternate Names ASD2; GATA binding protein 4; GATA-binding factor 4; GATA-binding protein 4; GATA4; MGC126629; TACHD; TOF; Transcription factor GATA-4; VSD1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 55 Source/Isotype: Rabbit IgG