CST, 37001S, ARHGAP29 (F6O5B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ARHGAP29. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, ARHGAP29 (F6O5B) Rabbit Monoclonal Antibody (CST #37001) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity ARHGAP29 (F6O5B) Rabbit Monoclonal Antibody recognizes endogenous levels of total ARHGAP29 protein. By western blot, this antibody detects a band of unknown origin at approximately 10 kDa. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly561 of human ARHGAP29 protein. Background Rho GTPase-activating protein 29 (ARHGAP29), also known as PARG1, suppresses Rho family GTPase signaling by promoting GTP hydrolysis and the inactive (GDP-bound) state. Transcription of ARHGAP29 is positively regulated by Yes-associated protein (YAP), destabilizing the actin cytoskeleton and promoting cell migration (1). Upregulated expression of ARHGAP29 is associated with invasion and proliferation in human prostate, breast, and kidney cancer cells, and may serve as a prognostic indicator in several human cancers (1-4). Mutation of the gene is associated with nonsyndromic cleft lip and/or palate (NSCL/P) in humans (5,6). Alternate Names ARHGAP29; PARG1; PTPL1-associated RhoGAP 1 (PARG1); PTPL1-associated RhoGAP protein 1; RHG29; Rho GTPase activating protein 29; Rho GTPase-activating protein 29; Rho-type GTPase-activating protein 29; RP11-255E17.1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 142 Source/Isotype: Rabbit IgG