CST, 3700S, beta-Actin (8H10D10) Mouse Monoclonal Antibody

Monoclonal Antibody for studying beta-Actin. Validated for WB,WB,WB,IHC,IF,F. Available in 3 sizes. Highly specific and rigorously validated in-house, beta-Actin (8H10D10) Mouse Monoclonal Antibody (CST #3700) is ready to ship. Product Usage Information Western Blotting: 1:1000 Fluorescent Western: 1:1000 Simple Western™: 1:10 - 1:50 Immunohistochemistry (Paraffin): 1:3000 - 1:12000 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600 Flow Cytometry (Fixed/Permeabilized): 1:800 - 1:3200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Fluorescent Western, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity beta-Actin (8H10D10) Mouse Monoclonal Antibody detects endogenous levels of total β-actin protein. Due to the high sequence identity between the cytoplasmic actin isoforms, β-actin and cytoplasmic γ-actin, this antibody may cross-react with cytoplasmic γ-actin. This antibody does not cross-react with α-skeletal, α-cardiac, α-vascular smooth, or γ-enteric smooth muscle isoforms. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey, Dog Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues of human β-actin. Background Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are ubiquitously expressed, controlling cell structure and motility (1). While all actin isoforms are highly homologous, cytoplasmic β- and γ-actin protein sequences differ by only four biochemically similar amino acids (2). For this reason, antibodies raised to β-actin may cross-react with γ-actin, and vice versa. α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin (3). The ARP2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments (3). Research studies have shown that actin is hyperphosphorylated in primary breast tumors (4). Cleavage of actin under apoptotic conditions has been observed and in cardiac and skeletal muscle, as shown in research studies (5-7). Actin cleavage by caspase-3 may accelerate ubiquitin/proteasome-dependent muscle proteolysis (7). Alternate Names ACTB; actin beta; actin, beta; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed; beta actin; beta cytoskeletal actin; Beta-actin; BRWS1; I(2)-actin; PS1TP5-binding protein 1; PS1TP5BP1 Specification REACTIVITY: H M R Hm Mk Dg SENSITIVITY: Endogenous MW (kDa): 45 Source/Isotype: Mouse IgG2b