CST, 3702S, GRB10 Antibody

Polyclonal Antibody for studying Grb10. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, GRB10 Antibody (CST #3702) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity GRB10 Antibody detects endogenous levels of isoform 1 of total GRB10 protein independent of phosphorylation. This antibody does not cross-react with GRB7 or GRB14. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues of human GRB10. Antibodies are purified by protein A and peptide affinity chromatography. Background The GRB7 family of adaptor proteins consist of GRB7, GRB10 and GRB14, which all contain an amino-terminal proline-rich SH3 binding domain, followed by PH, PBS, and SH2 domains. Each member of the family has several splice variants (1). It has been reported that GRB10 interacts with many receptor tyrosine kinases (RTKs) as well as downstream signal molecules including Raf, Akt, and Nedd4 (1,2). Although it was originally thought that GRB10 is exclusively phosphorylated at serine residues (3), Src kinase family members have been shown to phosphorylate GRB10 at Tyr67 (4). Alternate Names Grb-10; GRB-IR; GRB10; GRB10 adapter protein; GRB10 adaptor protein; GRBIR; growth factor receptor bound protein 10; Growth factor receptor-bound protein 10; Insulin receptor-binding protein Grb-IR; IRBP; KIAA0207; maternally expressed gene 1; MEG1; RSS Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 76 SOURCE: Rabbit