CST, 37095T, PRPF4 (F9Z1U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PRPF4. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, PRPF4 (F9Z1U) Rabbit Monoclonal Antibody (CST #37095) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PRPF4 (F9Z1U) Rabbit Monoclonal Antibody recognizes endogenous levels of total PRPF4 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala278 of human PRPF4 protein. Background PRPF4 is a U4/U6 snRNP-associated WD repeat-containing protein that is a critical regulator of the splicing process (1-3). PRPF4, PRPF3, and PPIH form a stable complex essential for proper formation and function of the U4/U6.U5 tri-snRNP (3,4). PRPF4 associates with kinetochores and can affect mitosis checkpoint control through MPS1, MAD1, and MAD2 (5). PRPF4 also has been shown to play a role in pluripotency and stem cell differentiation (6). Mutations in cause autosomal dominant retinitis pigmentosa (7,8). Furthermore, knockdown of PRPF4 has been shown to reduce colony formation and cell growth in breast cancer cells (9). Alternate Names hPrp4; HPRP4P; pre-mRNA processing factor 4; PRP4; PRP4 homolog; PRP4 pre-mRNA processing factor 4 homolog; PRP4 pre-mRNA processing factor 4 homolog (yeast); PRP4/STK/WD splicing factor; Prp4p; PRPF4; RP70; SNRNP60; U4/U6 small nuclear ribonucleoprotein Prp4; U4/U6 snRNP 60 kDa protein; WD splicing factor Prp4 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 60 Source/Isotype: Rabbit IgG