CST, 3710S, Phospho-ALK (Tyr1278) Antibody

Polyclonal Antibody for studying ALK (Tyr1278) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-ALK (Tyr1278) Antibody (CST #3710) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-ALK (Tyr1278) Antibody detects ALK only when phosphorylated at Tyr1278, which is equivalent to Tyr338 of NPM-ALK. This antibody also reacts with leukocyte tyrosine kinase (LTK) phosphorylated at Tyr672, and may cross-react with other activated protein tyrosine kinases including Bcr-Abl. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1278 of human ALK. Antibodies are purified by protein A and peptide affinity chromatography. Background Anaplastic lymphoma kinase (ALK) is a tyrosine kinase receptor for pleiotrophin (PTN), a growth factor involved in embryonic brain development (1-3). In ALK-expressing cells, PTN induces phosphorylation of both ALK and the downstream effectors IRS-1, Shc, PLCγ, and PI3 kinase (1). ALK was originally discovered as a nucleophosmin (NPM)-ALK fusion protein produced by a translocation (4). Investigators have found that the NPM-ALK fusion protein is a constitutively active, oncogenic tyrosine kinase associated with anaplastic lymphoma (4). Research literature suggests that activation of PLCγ by NPM-ALK may be a crucial step for its mitogenic activity and involved in the pathogenesis of anaplastic lymphomas (5). A distinct ALK oncogenic fusion protein involving ALK and echinoderm microtubule-associated protein like 4 (EML4) has been described in the research literature from a non-small cell lung cancer (NSCLC) cell line, with corresponding fusion transcripts present in some cases of lung adenocarcinoma. The short, amino-terminal region of the microtubule-associated protein EML4 is fused to the kinase domain of ALK (6-8). Phosphorylation of ALK on Tyr1278 was identified at Cell Signaling Technology (CST) using PhosphoScan , CST's LC-MS/MS platform for phosphorylation site discovery. Phosphorylation of ALK at Tyr1278 was observed in select carcinoma cell lines and in tumors (6). Alternate Names ALK; ALK receptor tyrosine kinase; ALK tyrosine kinase receptor; Anaplastic lymphoma kinase; anaplastic lymphoma receptor tyrosine kinase; CD246; CD246 antigen; mutant anaplastic lymphoma kinase; NBLST3 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 220 (ALK), 80 (NPM-ALK) SOURCE: Rabbit