CST, 37117T, EPHX2 (F9Z3M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying EPHX2. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, EPHX2 (F9Z3M) Rabbit Monoclonal Antibody (CST #37117) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity EPHX2 (F9Z3M) Rabbit Monoclonal Antibody recognizes endogenous levels of total EPHX2 protein. This antibody also recognizes a non-specific band of unknown origin at 140 kDa. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val380 of human EPHX2 protein. Background Soluble epoxide hydrolase 2 (EPHX2, sEH) is an enzyme member of the α/β hydrolase superfamily important for epoxide metabolism, especially those derived from fatty acids including epoxyeicosatrienoic acids (EETs). EPHX2 contains two functional domains connected by a proline-rich linker: a carboxy-terminal domain is responsible for its epoxide hydrolase activity and an amino-terminal phosphatase domain (1). EPHX2 is widely expressed in the central nervous system and peripheral tissue types, with highest expression found in the kidney and liver (1,2). Mutations in the gene have been linked to familial hypercholesterolemia due to its impact on plasma cholesterol levels (3). Studies have shown that suppression of EPHX2 activity through selective inhibitors promotes anti-inflammatory, cardioprotective, and neuroprotective effects through increased circulating EET levels, implicating EPHX2 as a therapeutic target for several disease conditions (4-6). Alternate Names ABHD20; Bifunctional epoxide hydrolase 2; CEH; Cytosolic epoxide hydrolase; Cytosolic epoxide hydrolase 2; EPHX2; Epoxide hydratase; Epoxide hydrolase 2; epoxide hydrolase 2, cytoplasmic; epoxide hydrolase 2, cytosolic; epoxide hydrolase, soluble; HYES; Lipid-phosphate phosphatase; SEH; Soluble epoxide hydrolase Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 60 Source/Isotype: Rabbit IgG