CST, 37135T, IRP2 (D6E6W) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying IRP2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, IRP2 (D6E6W) Rabbit Monoclonal Antibody (CST #37135) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity IRP2 (D6E6W) Rabbit Monoclonal Antibody recognizes endogenous levels of total IRP2 protein. This antibody does not recognize human IRP1. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro731 of human IRP2 protein. Background Iron regulatory proteins (IRPs; also known as IREBs) are RNA-binding proteins that recognize iron-responsive elements (IREs) and play an important role in maintaining iron homeostasis in mammalian cells. IREs are conserved cis-regulatory hairpin structures located within the 5' or 3' untranslated regions (UTRs) of target mRNAs. IRPs inhibit translation when bound to IREs within the 5' UTR of mRNA encoding for proteins involved in iron storage, export, and utilization. IRP binding to multiple IREs within the 3' UTR of transferin receptor 1 (TFR1) mRNA prevents its degradation, thereby augmenting translation of TFR1 and increasing iron uptake into cells (1-3). Dysregulation of IRPs has been associated with human cancers (4-6). In iron replete cells, FBXL5 targets IRP2 for degradation. Under iron deficiency and/or hypoxic conditions, FBXL5 is destabilized, resulting in IRP2 accumulation and interaction with IRE-containing mRNA (7). Alternate Names ACO3; FLJ23381; IRE-BP 2; IRE-BP2; IREB2; Iron regulatory protein 2; iron responsive element binding protein 2; iron-responsive element binding protein 2; Iron-responsive element-binding protein 2; IRP2; IRP2AD; NDCAMA Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 105 Source/Isotype: Rabbit IgG