CST, 37215SF, PRAS40 (D23C7) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying PRAS40. Validated for Western Blotting,Immunohistochemistry (Paraffin),ELISA+. Highly specific and rigorously validated in-house, PRAS40 (D23C7) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #37215) is ready to ship. Product Usage Information This product is the carrier free version of product #2691. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 2691 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity PRAS40 (D23C7) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of total PRAS40 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro185 of human PRAS40 protein. Background Many growth factors and hormones induce the phosphoinositide 3-kinase signaling pathway, which results in the activation of downstream effector proteins such as the serine/threonine kinase Akt (1,2). One known Akt substrate is a 40 kDa, proline-rich protein (PRAS40) that binds to 14-3-3 proteins (2). PRAS40 also binds mTOR to transduce Akt signals to the mTOR complex. Inhibition of mTOR signaling stimulates PRAS40 binding to mTOR, which in turn inhibits mTOR activity (3). PRAS40 interacts with raptor in mTOR complex 1 (mTORC1) in insulin-deprived cells and inhibits the activation of the mTORC1 pathway mediated by the cell cycle protein Rheb. Phosphorylation of PRAS40 by Akt at Thr246 relieves PRAS40 inhibition of mTORC1 (4). mTORC1 in turn phosphorylates PRAS40 at Ser183 (5). Alternate Names 40 kDa proline-rich AKT substrate; AKT1 substrate 1; AKT1 substrate 1 (proline rich); AKT1S1; AKTS1; Lobe; PRAS40; Proline-rich AKT1 substrate 1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 40 Source/Isotype: Rabbit IgG