CST, 3722S, Pin1 Antibody

Polyclonal Antibody for studying Pin1. Validated for Western Blotting. Highly specific and rigorously validated in-house, Pin1 Antibody (CST #3722) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Pin1 Antibody detects endogenous levels of total Pin1 protein. This antibody does not cross-react with other proteins. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal residues of human Pin1. Antibodies are purified by protein A and peptide affinity chromatography. Background Pin1, a member of the parvulin family of peptidyl-prolyl isomerases (PPIase), has been implicated in the G2/M transition of the mammalian cell cycle (1-6). Pin1 is a small (18 kDa) protein with two distinct functional domains: an amino-terminal WW domain and a carboxy-terminal PPlase domain. Pin1 interacts with several mitotic phosphoproteins, including Plk1, cdc25C and cdc27, and is thought to act as a phosphorylation-dependent PPlase for these target molecules (7-9). Alternate Names DOD; Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1; Peptidyl-prolyl cis-trans isomerase Pin1; peptidyl-prolyl cis/trans isomerase, NIMA-interacting; peptidylprolyl cis/trans isomerase, NIMA-interacting 1; PIN1; PPIase Pin1; prolyl isomerase; protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1; protein (peptidylprolyl cis/trans isomerase) NIMA-interacting 1; protein interacting with never in mitosis A1; Rotamase Pin1; UBL5 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 18 SOURCE: Rabbit