CST, 37270S, Reg4 (E7Z7S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying REG4 mouse. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Reg4 (E7Z7S) Rabbit Monoclonal Antibody (CST #37270) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Reg4 (E7Z7S) Rabbit Monoclonal Antibody recognizes endogenous levels of total Reg4 protein. In some mouse tissues, such as liver, non-specific bands of different molecular weights, but of unknown identity may be observed. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of murine Reg4 protein. Background Reg4 (regenerating islet-derived protein 4) is a member of a multigene family of Reg proteins (1). Reg proteins are secreted glycoproteins belonging to the calcium (C-type) dependent lectin superfamily, although they can in fact bind polysaccharides, mannan, and heparin in the absence of calcium (2,3). Reg4 is expressed in the gastrointestinal (GI) tract, in normal colon mucosa, and is upregulated in colon adenocarcinoma, pancreatic cancer, gastric adenocarcinoma, and inflammatory bowel disease (Crohn's disease and ulcerative colitis) (4,5). It has recently been shown that Reg4 marks a population of deep secretory cells at the bottom of the crypts in the colon. In the upper gastrointestinal tract, Paneth cells support the Lgr5+ positive stem cells that reside at the base of deep crypts by providing signals such as Wnt3, EGF, and Notch ligands for stem cell maintenance. No Paneth cells, however, exist in the colon crypts. It is proposed that the Reg4+ population serves as an analogous support system for the LGR5+ positive stem cell population in the colon (1,6). Alternate Names 2010002L15Rik; GISP; REG-4; Reg4; regenerating islet-derived family, member 4; Regenerating islet-derived protein 4; RELP Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 14, 22 Source/Isotype: Rabbit IgG