CST, 3729S, OSR1 Antibody

Polyclonal Antibody for studying OSR1. Validated for Western Blotting. Highly specific and rigorously validated in-house, OSR1 Antibody (CST #3729) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity OSR1 Antibody detects endogenous levels of total OSR1 protein. This antibody does not cross-react with SPAK or other members of the GCK family. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues at the amino terminus of human OSR1. Antibodies are purified by protein A and peptide affinity chromatography. Background SPAK (STE20/SPS1-related Pro/Ala-rich kinase) and OSR1 (oxidative stress responsive 1) are members of the GCK family of serine/threonine kinases. Overexpression and studies demonstrate that SPAK is able to activate p38 MAP kinase, indicating a possible role for SPAK in the stress response (1). Yeast two-hybrid screening revealed that SPAK and OSR1 bind to Na-K-2Cl cotransporters NKCC1 and NKCC2 and K-Cl cotransporter KCC3 (2). WNK1 and WNK4 phosphorylate SPAK at Thr243/247 and Ser380 (3-5). Similarly, WNK1 and WNK4 phosphorylate OSR1 at Thr185 and Ser315 (3,4). Phosphorylation at these sites stimulates SPAK and OSR1 activity, leading to NKCC1 phosphorylation and enhanced NKCC1 activity (3-5). SPAK is also phosphorylated at Ser311 by PKCθ in response to T cell activation. Substitution of Ser311 with Ala or specific siRNA knockdown of SPAK dramatically reduces TCR/CD28-induced AP-1 activation, suggesting SPAK is involved in T cell signaling as well (6). Alternate Names KIAA1101; OSR1; oxidative stress responsive 1; oxidative stress responsive kinase 1; Oxidative stress-responsive 1 protein; oxidative-stress responsive 1; OXSR1; Serine/threonine-protein kinase OSR1 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 60 SOURCE: Rabbit