CST, 3731S, Caspr2 Antibody

Polyclonal Antibody for studying Caspr2. Validated for Western Blotting. Highly specific and rigorously validated in-house, Caspr2 Antibody (CST #3731) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Caspr2 Antibody detects endogenous levels of total Caspr2 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide sourrounding Glu1320 of human Caspr2. Antibodies are purified by protein A and peptide affinity chromatography. Background ontactin- sociated otein 2 (Caspr2) is a type I transmembrane protein and member of the neurexin superfamily that mediates nervous system cell-cell interactions through the Neurexin IV-Caspr-Paranodin (NCP) complex (1). A multiprotein complex consisting of TAG-1, Caspr2, K channel, PSD95 and protein 4.1B mediates the molecular interactions at the juxtaparanodal region of myelinated axons, with homophilic TAG-1 interactions mediating the binding of this complex to glia (2,3). Caspr2 protein localizes to juxtaparanodal regions of myelinated axons where it forms a -complex with the immunoglobulin-like cell adhesion molecule TAG-1. Caspr2 also binds to Shaker K channels Kv1.1, Kv1.2, and their Kvβ2 subunit. A PDZ domain at the Caspr2 carboxy terminus mediates the Caspr2-K channel association. Caspr2 is required for proper K channel localization, as Caspr2 deletion causes the redistribution of channels along the internodes (1-3). Furthermore, Caspr2 binds to protein 4.1B and connects the protein complex to the axonal cytoskeleton (4). Mutations in the Caspr2 gene have been linked to focal epilepsy, cortical dysplasia and Gilles de la Tourette syndrome (5,6). Alternate Names AUTS15; CASPR2; CDFE; Cell recognition molecule Caspr2; CNTNAP2; CNTP2; contactin associated protein 2; contactin associated protein like 2; contactin associated protein-like 2; contactin-associated protein 2; Contactin-associated protein-like 2; DKFZp781D1846; homolog of Drosophila neurexin IV; KIAA0868; NRXN4; PTHSL1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 150 SOURCE: Rabbit