CST, 3732S, GP130 Antibody

Polyclonal Antibody for studying GP130. Validated for Western Blotting. Highly specific and rigorously validated in-house, GP130 Antibody (CST #3732) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity GP130 Antibody detects endogenous levels of total GP130 protein independent of phosphorylation. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human GP130. Antibodies are purified by protein A and peptide affinity chromatography. Background GP130 is a signal-transducing subunit shared by the receptors for the interleukin-6 (IL-6) family of cytokines (1-3). The binding of a ligand to its receptor induces GP130 dimerization, leading to Jak tyrosine kinase activation and GP130 tyrosine phosphorylation. These events lead to the activation of multiple signal-transduction pathways, such as the Stat, Ras-MAPK, and PI3 kinase pathways, whose activation is controlled by distinct regions of GP130 (4-7). Alternate Names CD130; CD130 antigen; CDw130; gp130; gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form; gp130 transducer chain; GP130-RAPS; gp130, oncostatin M receptor; HIES4; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6R-beta; IL-6RB; IL6R-beta; IL6RB; IL6ST; IL6ST nirs variant 3; interleukin 6 signal transducer; interleukin 6 signal transducer (gp130, oncostatin M receptor); interleukin receptor beta chain; Interleukin-6 receptor subunit beta; Interleukin-6 signal transducer; Membrane glycoprotein 130; membrane glycoprotein gp130; oncostatin M receptor; Oncostatin-M receptor subunit alpha; sGP130 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 130 SOURCE: Rabbit