CST, 37344S, NPRL2 (D8K3X) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NPRL2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, NPRL2 (D8K3X) Rabbit Monoclonal Antibody (CST #37344) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity NPRL2 (D8K3X) Rabbit Monoclonal Antibody recognizes endogenous levels of total NPRL2 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala211 of human NPRL2 protein. Background The mTORC1 kinase complex plays a critical role in cell growth regulation (1, 2). mTORC1 activity is modulated by cellular and environmental factors (e.g., energy levels, growth factors, and amino acids) (3, 4). Amino acid sensing is mediated through several protein complexes, including GATOR (GAP Activity TOward Rags). GATOR is composed of two protein subcomplexes (GATOR1 and GATOR2) that function in opposing fashion to regulate mTORC1 activity. NPRL2 was identified as a component of the GATOR1 subcomplex (also containing DEPDC5 and NPRL3) that functions to negatively regulate mTORC1 activity through activation of RagA and RagB GTPases (5). Conversely, the GATOR2 subcomplex (containing Mios, WDR24, WDR59, Seh1L, and Sec13) positively regulates mTORC1 activity (5). In addition, NPRL2, also known as TUSC4 (tumor suppressor candidate 4), has been shown to prevent the degradation of tumor suppressor BRCA1. Overexpression of TUSC4 (NPRL2) protein inhibits proliferation of breast cancer cells (6). Alternate Names 2810446G01Rik; FFEVF2; G21 protein; GATOR complex protein NPRL2; GATOR1 complex protein NPRL2; Gene 21 protein; homologous to yeast nitrogen permease (candidate tumor suppressor); Nitrogen permease regulator 2-like protein; nitrogen permease regulator-like 2 (S. cerevisiae); NPR2; NPR2 like, GATOR1 complex subunit; NPR2-like protein; NPR2L; NPRL2; Tumor suppressor candidate 4; TUSC4 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 41 Source/Isotype: Rabbit IgG