CST, 37403S, BACH2 (E3N3K) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying BACH2 mouse. Validated for Western Blotting,Immunoprecipitation,Flow Cytometry (Fixed/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, BACH2 (E3N3K) Rabbit Monoclonal Antibody (CST #37403) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Flow Cytometry (Fixed/Permeabilized): 1:1000 - 1:4000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity BACH2 (E3N3K) Rabbit Monoclonal Antibody recognizes endogenous levels of total BACH2 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the central region of mouse BACH2 protein. Background The transcription regulator BTB and CNC homolog 2 (BACH2) is a bZIP domain-containing transcriptional repressor that dimerizes with MafK and binds Maf recognition elements (MAREs) to regulate transcription (1,2). BACH2 is part of a network of transcription factors that controls the transition of activated B cells into either antibody-producing plasma cells or memory B cells (3-5). Plasma cell differentiation requires the transcription factor Blimp1 (6). BACH2 suppresses expression of Blimp1 in activated B cells, which delays plasma cell differentiation and allows time for class switch recombination and somatic hypermutation (3-5). Genome-wide association studies have linked the genetic locus containing BACH2 to several immune-related disorders, including type 1 diabetes, celiac disease, Crohn's disease, and the skin condition known as vitiligo (7-10). Alternate Names Bach2; Bach2 protein; BTB and CNC homolog 2; BTB and CNC homology 2; BTB and CNC homology, basic leucine zipper transcription factor 2; E030004N02Rik; Transcription regulator protein BACH2; Transcription regulator protein BACH2 (BTB and CNC homolog 2) Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 130 Source/Isotype: Rabbit IgG