CST, 3741S, Cyclin D2 (D52F9) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Cyclin D2. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Cyclin D2 (D52F9) Rabbit Monoclonal Antibody (CST #3741) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Cyclin D2 (D52F9) Rabbit Monoclonal Antibody detects endogenous levels of total cyclin D2 protein. This antibody does not cross-react with other family members. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of cyclin D2. Background Activity of the cyclin-dependent kinases CDK4 and CDK6 is regulated by T-loop phosphorylation, by the abundance of their cyclin partners (the D-type cyclins), and by association with CDK inhibitors of the Cip/Kip or INK family of proteins (1). The inactive ternary complex of cyclin D/CDK4 and p27 Kip1 requires extracellular mitogenic stimuli for the release and degradation of p27 concomitant with a rise in cyclin D levels to affect progression through the restriction point and Rb-dependent entry into S-phase (2). The active complex of cyclin D/CDK4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression (3). Levels of cyclin D protein drop upon withdrawal of growth factors through downregulation of protein expression and phosphorylation-dependent degradation (4). Alternate Names CCND2; cyclin D2; G1/S-specific cyclin D2; G1/S-specific cyclin-D2; KIAK0002; MGC102758; MPPH3 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 31 Source/Isotype: Rabbit IgG