CST, 37458S, Phospho-Lck (Tyr505) (E3Z5E) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Lck (Tyr505) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Lck (Tyr505) (E3Z5E) Rabbit Monoclonal Antibody (CST #37458) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-Lck (Tyr505) (E3Z5E) Rabbit Monoclonal Antibody recognizes endogenous levels of Lck protein only when phosphorylated at Tyr505. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr505 of human Lck protein. Background The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr530 in the carboxy-terminal tail by Csk renders the enzyme less active (2). Lck is essential for T-lymphocyte activation and differentiation (3,4). Phosphorylation of Tyr505 in the carboxy-terminal tail of Lck downregulates its catalytic activity, while phosphorylation of Tyr394 leads to an increase in Lck activity (5). Alternate Names IMD22; LCK; LCK proto-oncogene, Src family tyrosine kinase; Leukocyte C-terminal Src kinase; LSK; Lymphocyte cell-specific protein-tyrosine kinase; lymphocyte-specific protein tyrosine kinase; p56-LCK; p56(LSTRA) protein-tyrosine kinase; p56lck; pp58lck; Protein YT16; Proto-oncogene Lck; proto-oncogene tyrosine-protein kinase LCK; T cell-specific protein-tyrosine kinase; T-lymphocyte specific protein tyrosine kinase p56lck; Tyrosine-protein kinase Lck; YT16 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 56 Source/Isotype: Rabbit IgG