CST, 3750S, Integrin alpha6 Antibody

Polyclonal Antibody for studying Integrin Alpha 6. Validated for Western Blotting. Highly specific and rigorously validated in-house, Integrin alpha6 Antibody (CST #3750) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Integrin alpha6 Antibody detects endogenous levels of total integrin α6 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding residues surrounding Gly571 of human integrin α6. Antibodies are purified by peptide affinity chromatography. Background Integrins are α/β heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and 8 β subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with the extracellular environment (inside-out signaling) (1,2). Integrin α6 is a 120 kDa protein with two splice variants, integrin α6, 6A and 6B (3), which function as receptors for laminins on the basal membrane to mediate cellular adhesion events (4-6). α6 integrins have been shown to play an important role in hematopoietic stem and progenitor cell homing to the bone marrow. Alternate Names Alpha6p; CD49 antigen-like family member F; CD49f; DKFZp686J01244; FLJ18737; Integrin alpha-6; Integrin alpha-6 heavy chain; Integrin alpha-6 light chain; integrin alpha6B; integrin subunit alpha 6; integrin, alpha 6; ITA6; ITGA6; ITGA6B; Processed integrin alpha-6; VLA-6 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 150, 125 SOURCE: Rabbit