CST, 37517S, GUCY2C (E6I2U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying GUCY2C. Validated for Western Blotting,Immunoprecipitation,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, GUCY2C (E6I2U) Rabbit Monoclonal Antibody (CST #37517) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 92931 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin) Specificity / Sensitivity GUCY2C (E6I2U) Rabbit Monoclonal Antibody recognizes endogenous levels of total GUCY2C protein. Non-specific staining was observed in acinar cells of the salivary gland and keratinized epithelium of the skin by immunohistochemistry. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys1046 of human GUCY2C protein. Background Guanylyl cyclase C (GUCY2C) is a type I transmembrane glycoprotein whose expression in normal tissue is largely restricted to the apical membranes of polarized epithelial cells of the intestine (1,2). The guanylyl cyclase activity within the intracellular domain of GUCY2C is activated by binding exogenous bacterial enterotoxins and the endogenous peptide hormone agonists, guanylin and uroguanylin, which facilitates production of the second messenger cGMP (3-5). GUCY2C signaling plays a critical role in regulating the metabolism, proliferation, and differentiation of intestinal epithelial cells. These processes are critical for epithelial barrier renewal, water, and ion balance (6-8). Research studies have shown that the GUCY2C signaling axis functions to suppress intestinal tumorigenesis, in part, by restraining AKT activity (9,10). Lesions in the GUCY2C signaling axis that frequently contribute to cellular transformation are associated with a loss of expression of its endogenous ligands, which promotes a compensatory increase in GUCY2C expression. Indeed, GUCY2C expression is maintained in a high percentage of primary and metastatic human colorectal tumors, which has raised interest in therapeutically targeting this receptor (11,12). Alternate Names DIAR6; GC-C; guanylate cyclase 2C; guanylate cyclase 2C (heat stable enterotoxin receptor); Guanylyl cyclase C; GUC2C; GUCY2C; Heat-stable enterotoxin receptor; hSTAR; Intestinal guanylate cyclase; MECIL; MUCIL; STA receptor; STAR Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 145, 130 Source/Isotype: Rabbit IgG