CST, 37562S, hnRNP L Antibody

Polyclonal Antibody for studying hnRNP L. Validated for Western Blotting. Highly specific and rigorously validated in-house, hnRNP L Antibody (CST #37562) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity hnRNP L Antibody recognizes endogenous levels of total hnRNP L protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His313 of human hnRNP L protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Heterogeneous nuclear ribonucleoprotein L (hnRNP L) belongs to a family of RNA binding multiprotein complexes (hnRNP proteins) that facilitate pre-mRNA processing and transport of mRNA from the nucleus to cytoplasm (1-3). hnRNP L binds to CA repeat elements to regulate splicing and RNA stability (4-6). Binding of hnRNP L has been shown to occur in response to stress stimuli to regulate conformational change and subsequent translation of mRNAs (7). hnRNPL can promote apoptosis by enhancing translation of p53 mRNA (8) . In the context of tumor progression, hnRNPL has been shown to bind and modify translation of critical regulators of cell cycle and apoptosis (9,10). Alternate Names epididymis secretory sperm binding protein; FLJ35509; Heterogeneous nuclear ribonucleoprotein L; hnRNP L; hnRNP-L; HNRNPL; HNRPL; P/OKcl.14 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 60, 66 SOURCE: Rabbit