CST, 37565T, Phospho-VASP (Ser239) (E9L7Y) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying VASP (Ser239) phosphate. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-VASP (Ser239) (E9L7Y) Rabbit Monoclonal Antibody (CST #37565) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-VASP (Ser239) (E9L7Y) Rabbit Monoclonal Antibody recognizes endogenous levels of VASP protein only when phosphorylated at Ser239. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser239 of human VASP protein. Background Vasodilator-stimulated phosphoprotein (VASP) was originally characterized as a substrate of both cGMP- and cAMP-dependent kinases (PKG and PKA, or cGPK and cAPK, respectively) (1). It is now believed that VASP belongs to the Ena/VASP family of adaptor proteins linking the cytoskeletal system to the signal transduction pathways and that it functions in cytoskeletal organization, fibroblast migration, platelet activation, and axon guidance (2,3). Three phosphorylation sites, Ser157, Ser239, and Thr278, have been identified. Ser239 is the major PKG phosphorylation site, while Ser157 is the major PKA phosphorylation site (4). Evidence suggests that VASP phosphorylation reduces its association with actin and has a negative effect on actin polymerization (5). Phosphorylation at Ser239 of VASP is a useful marker for monitoring PKG activation and signaling (6,7). Alternate Names vasodilator stimulated phosphoprotein; Vasodilator-stimulated phosphoprotein; VASP Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 48, 50 Source/Isotype: Rabbit IgG