CST, 3760S, Phospho-IP3 Receptor (Ser1756) Antibody

Polyclonal Antibody for studying IP3R1 (Ser1764) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-IP3 Receptor (Ser1756) Antibody (CST #3760) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-IP3 Receptor (Ser1756) Antibody detects endogenous levels of IP3 Receptor only when phosphorylated at Ser1756. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser1756 of rat IP3 receptor. Antibodies are purified by peptide affinity chromatography. Background Inositol 1,4,5-triphosphate receptor, also known as IP3R or InsP3R, is a member of the intracellular calcium release channel family and is located in the endoplasmic reticulum. IP3R functions as a Ca release channel for intracellular stores of calcium ions. There are three types of IP3 receptors (IP3R1, 2, and 3) that require the second messenger inositol 1,4,5-triphosphate (IP3) for activation (1). Four individual subunits homo- or hetero-oligomerize to form the receptor's functional channel (2). Phosphorylation of IP3R1 at Ser1756 by cyclic AMP-dependent protein kinase A (PKA) regulates the sensitivity of IP3R1 to IP3 and may be a mode of regulation for Ca release (3,4). IP3R1-mediated Ca release appears to have an effect on the induction of long term depression (LTD) in Purkinje cells (5). Alternate Names ACV; CLA4; DKFZp313E1334; DKFZp313N1434; inositol 1,4,5-triphosphate receptor, type 1; Inositol 1,4,5-trisphosphate receptor type 1; INSP3R1; IP3 receptor; IP3 receptor isoform 1; IP3R; IP3R 1; IP3R1; ITPR1; PPP1R94; protein phosphatase 1, regulatory subunit 94; SCA15; SCA16; SCA29; Type 1 inositol 1,4,5-trisphosphate receptor; Type 1 InsP3 receptor Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 320 SOURCE: Rabbit