CST, 3766S, Thymidylate Synthase Antibody

Polyclonal Antibody for studying Thymidylate Synthase. Validated for Western Blotting. Highly specific and rigorously validated in-house, Thymidylate Synthase Antibody (CST #3766) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Thymidylate Synthase Antibody detects endogenous levels of total thymidylate synthase protein. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human thymidylate synthase. Antibodies are purified using protein A and peptide affinity chromatography. Background The methylation of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP) is an essential step in the formation of thymine nucleotides (1,2, reviewed in 3). This process is catalyzed by thymidylate synthase (TS or TYMS), a homodimer composed of two 30 kDa subunits. TS is an intracellular enzyme that provides the sole source of thymidylate, making it a required enzyme in DNA biosynthesis with activity highest in proliferating cells (1). Being the exclusive source of dTMP, investigators have concluded that TS is also an important target for anticancer agents such as 5-fluorouracil (5-FU) (1-5). 5-FU acts as a TS inhibitor and is active against solid tumors such as colon, breast, head, and neck. Research studies have demonstrated that patients with metastases expressing lower levels of TS have a higher response rate to treatment with 5-FU than patients with tumors that have increased levels of TS (5). Researchers continue to investigate TS expression in different types of cancers (6-10). Alternate Names HST422; MGC88736; Thymidylate synthase; thymidylate synthetase; TMS; TS; TSase; TYMS; TYSY Specification REACTIVITY: H M R Hm Mk SENSITIVITY: Endogenous MW (kDa): 30 SOURCE: Rabbit