CST, 3775S, Jak3 Antibody

Polyclonal Antibody for studying Jak3. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Jak3 Antibody (CST #3775) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Jak3 Antibody detects endogenous levels of total Jak3 protein. No cross-reactivity was detected with other family members at physiological conditions. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues at the carboxy-terminus of Jak3. Antibodies are purified by protein A and peptide affinity chromatography. Background Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains. Alternate Names JAK-3; JAK3; JAK3_HUMAN; JAKL; Janus kinase 3; Janus kinase 3 (a protein tyrosine kinase, leukocyte); L-JAK; Leukocyte janus kinase; LJAK; Tyrosine-protein kinase JAK3 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 115 SOURCE: Rabbit