CST, 37765T, Granzyme M (E7B4W) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Granzyme M. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Granzyme M (E7B4W) Rabbit Monoclonal Antibody (CST #37765) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Granzyme M (E7B4W) Rabbit Monoclonal Antibody recognizes endogenous levels of total Granzyme M protein. This antibody does not cross-react with human Granzyme A, B, H, or K proteins. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala141 of human Granzyme M protein. Background Granzymes are a family of serine proteases expressed by cytotoxic T lymphocytes and natural killer (NK) cells and are key components of immune responses to pathogens and transformed cells (1). Granzymes are synthesized as zymogens and are processed into mature enzymes by cleavage of a leader sequence. They are released by exocytosis in lysosome-like granules containing perforin, a membrane pore-forming protein. Granzyme B has the strongest apoptotic activity of all the granzymes as a result of its caspase-like ability to cleave substrates at aspartic acid residues thereby activating procaspases directly and cleaving downstream caspase substrates (2,3). Granzyme M prefers to cleave after a Methionine or Leucine residue, and proteolyzes a restricted set of macromolecular substrates (4-7). Granzyme M is highly expressed in NK, NKT, γδ T cells, and in differentiated effector CD8  T cells, suggesting a role for Granzyme M in both innate and adaptive immunity (4-11). After perforin-mediated entry into a target cell, various reports show Granzyme M can induce apoptosis and tumor cell death, stimulate LPS-mediated inflammation, or inhibit viral replication (4,11-15). Alternate Names GRAM; Granzyme M; granzyme M (lymphocyte met-ase 1); GZMM; Hu-Met-1; LMET1; lymphocyte met-ase 1; Met-1 serine protease; Met-ase; MET1; Natural killer cell granular protease Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 25-30 Source/Isotype: Rabbit IgG