CST, 3776S, Phospho-Jak2 (Tyr1007/1008) (C80C3) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Jak2 (Tyr1007/Tyr1008) phosphate. Validated for Western Blotting,Fluorescent Western. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Jak2 (Tyr1007/1008) (C80C3) Rabbit Monoclonal Antibody (CST #3776) is ready to ship. Product Usage Information Western Blotting: 1:1000 Fluorescent Western: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Fluorescent Western Specificity / Sensitivity Phospho-Jak2 (Tyr1007/1008) (C80C3) Rabbit Monoclonal Antibody detects endogenous levels of Jak2 when phosphorylated at Tyr1007/1008. This antibody can also detect single phosphorylation at either 1007 or 1008. This antibody may cross-react with phospho-Jak1 and phospho-Tyk2. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to a region surrounding Tyr1007/1008 of human Jak2. Background Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains. Alternate Names JAK-2; JAK2; Janus kinase 2; Janus kinase 2 (a protein tyrosine kinase); JTK10; Tyrosine-protein kinase JAK2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 125 Source/Isotype: Rabbit IgG