CST, 37775SF, IL4R (F6C4Z) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying IL4R mouse. Validated for Western Blotting,Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, IL4R (F6C4Z) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #37775) is ready to ship. Product Usage Information This product is the carrier free version of product #56538. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 56538 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity IL4R (F6C4Z) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total IL4R protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse IL4R protein. Background In the immune system, interleukin-4 (IL-4) controls B cell growth, survival, and maturation, in addition to proliferation and differentiation of the Th2 subset of T cells. IL-4 is a cytokine secreted by activated T cells, basophils, and mast cells (1,2). It acts through the IL-4 receptor (IL4R), leading to tyrosine phosphorylation and activation of the Stat6 transcription factor (3). IL4R is a transmembrane ligand binding protein composed of a heterodimer alpha and gamma receptor complex (4,5). IL4R has been shown to bind both IL-4 and interleukin-13 (IL-13), regulating immunoglobulin E (IgE) and chemokine production during allergic inflammation (6). In addition to its role in regulating immune response, signaling through IL4R is also implicated in diseases such as asthma, allergy, and cancer, making it a potent target for various therapies (7-9). Increased IL4R expression has been observed in tumors (9,10), and several mutations in IL4R are associated with metastatic cancers, underscoring its importance in cancer progression and as a target for chemotherapy development (10,11). Alternate Names CD124; IL-4 receptor alpha chain; IL-4 receptor subunit alpha; IL-4-binding protein; IL-4R subunit alpha; IL-4R-alpha; IL-4RA; IL4-BP; Il4r; Il4ra; interleukin 4 receptor, alpha; Interleukin-4 receptor subunit alpha; MGC118473; sIL4Ralpha/prot; Soluble IL-4 receptor subunit alpha; Soluble IL-4R-alpha; Soluble interleukin-4 receptor subunit alpha Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 75-80, 120 Source/Isotype: Rabbit IgG