CST, 37783S, NUFIP1 Antibody

Polyclonal Antibody for studying NUFIP1. Validated for Western Blotting. Highly specific and rigorously validated in-house, NUFIP1 Antibody (CST #37783) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity NUFIP1 Antibody recognizes endogenous levels of total NUFIP1 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human NUFIP1 protein. Antibodies are purified by peptide affinity chromatography. Background NUFIP1 (nuclear FMRP interacting protein 1) was identified as an RNA-binding protein shuttling between the nucleus and cytoplasm, and it associates with ribosomes in functional synaptoneurosomes (1). Reports have shown that NUFIP1 can function as a receptor for starvation-induced ribophagy to maintain protein homeostasis (2). Ribophagy is a selective form of autophagy targeting ribosomes (reviewed in 3). Lysosomal levels of NUFIP1 were increased upon inhibition of autophagy. Ribophagy was dependent on the ability of ribosome-associated NUFIP1 to interact with LC3B via a conserved LC3-interacting region (LIR) domain and recruit ribosomes to the autophagosome (2). Loss of NUFIP1 or its LC3B binding ability inhibited cell survival following starvation (2). Alternate Names bA540M5.1; FMR1-interacting protein NUFIP1; nuclear FMR1 interacting protein 1; Nuclear FMRP-interacting protein 1; nuclear fragile X mental retardation protein interacting protein 1; nuclear fragile X mental retardation protein-interacting protein 1; Nuclear fragile X mental retardation-interacting protein 1; NUFIP; NUFIP1; NUFIP1, FMR1 interacting protein 1; NUFP1; Rsa1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 75 SOURCE: Rabbit