CST, 3782S, GCK Antibody

Polyclonal Antibody for studying GCK/MAP4K2. Validated for Western Blotting. Highly specific and rigorously validated in-house, GCK Antibody (CST #3782) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity GCK Antibody detects endogenous levels of total GCK protein. This antibody does not cross-react with HPK or other members of the GCK family. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser380 of human GCK. Antibodies are purified by protein A and peptide affinity chromatography. Background Germinal center kinase (GCK) is the founding member of the GCK family, a group of serine/threonine kinases homologous to the yeast Ste20 kinase. GCK is activated by TNF and associated with TRAF2 (TNF receptor-associated factor 2) and MEKK1, thereby activating the SAPK/JNK pathway (1,2). GCK does not significantly phosphorylate MEKK1, instead it enhances MEKK1 oligomerization and autophosphorylation (3). GCK binds and activates MLK3, indicating that GCK may have other effectors (3). Alternate Names B lymphocyte serine/threonine protein kinase; B lymphocyte serine/threonine-protein kinase; BL44; GC kinase; GCK; Germinal center kinase; germinal centre kinase (GC kinase); M4K2; MAP4K2; MAPK/ERK kinase kinase kinase 2; MEK kinase kinase 2; MEKKK 2; Mitogen-activated protein kinase kinase kinase kinase 2; Rab8 interacting protein; Rab8-interacting protein; RAB8IP Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 91 SOURCE: Rabbit