CST, 37851S, Phospho-IFNAR1 (Ser535) (F4V7F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying IFNAR1 (Ser535) phosphate. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-IFNAR1 (Ser535) (F4V7F) Rabbit Monoclonal Antibody (CST #37851) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-IFNAR1 (Ser535) (F4V7F) Rabbit Monoclonal Antibody recognizes endogenous levels of IFNAR1 protein only when phosphorylated at Ser535. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser535 of human IFNAR1 protein. Background Interferon alpha/beta receptor 1 (IFNAR1) is one of the two protein subunits that comprise the type I interferon (IFN-I) surface receptor (1-4). IFNAR1 and IFNAR2 belong to the class II helical cytokine receptor (hCR) family, are ubiquitously expressed, and contain highly glycosylated extracellular domains, leading to high molecular weight by western blot (1-4,8). IFN-Is bind the IFN receptor, leading to the dimerization of IFNAR2 and IFNAR1 subunits, and the activation of Janus family kinase (JAK) and signal transducer and activator of transcription (STAT) signaling pathways (2-5). All IFNαs bind IFNAR1 with a low affinity, and only IFNβ binds IFNAR1 with a relatively higher affinity (4,5). IFN-I signaling mediates various cellular responses, including antiviral responses, immunomodulatory responses, and antiproliferative effects (5). IFN-I signaling is being investigated as a therapeutic target for cancer, autoimmunity, sepsis, and viral infection (6,7). Phosphorylation of IFNAR1, triggered by ligand binding and ligand-independent means, plays an important role in regulating IFN-I signaling. IFNAR1 phosphorylation at Ser535 leads to endocytosis and IFNAR1 degradation, leading to the decreased sensitivity of cells to subsequent cytokine stimulation (9-11). PKD2 phosphorylates IFNAR1 at Ser535 and is dependent on TYK2 catalytic activity in the ligand-induced pathway (10). In the ligand-independent pathway, IFNAR1 can be phosphorylated at Ser535 by CK1a (12). Alternate Names alpha-type antiviral protein; AVP; beta-type antiviral protein; CRF2-1; Cytokine receptor class-II member 1; Cytokine receptor family 2 member 1; IFN-alpha-REC; IFN-alpha/beta receptor 1; IFN-R-1; IFNAR; IFNAR1; IFNBR; IFRC; INAR1; interferon (alpha, beta and omega) receptor 1; interferon alpha and beta receptor subunit 1; Interferon alpha/beta receptor 1; interferon receptor 1; interferon-alpha/beta receptor alpha chain; interferon-beta receptor 1; Type I interferon receptor 1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 110 Source/Isotype: Rabbit IgG