CST, 37872T, Ly-6C (E7L3S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying LY6C1 mouse/LY6C2 mouse. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Ly-6C (E7L3S) Rabbit Monoclonal Antibody (CST #37872) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Ly-6C (E7L3S) Rabbit Monoclonal Antibody recognizes endogenous levels of total Ly-6C1 and Ly-6C2 proteins. This antibody also cross-reacts with an unidentified protein of 90 kDa in some cell lines. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp86 of human Ly-6C1 and Ly-6C2 proteins. Background The Ly-6 complex is a series of genes found on chromosome 15. These genes code for a number of different proteins that can be used as surface markers. The family members vary in their biologic expression and have been shown to be involved in cell signaling and cell adhesion (1). The structure of these proteins includes a motif known as the LU domain that has three loops comprised of disulfide bonds. These bonds are formed by 8 to 10 cysteines that can cause differences in the length of the loops as well as the sequences at each tip (2,4). There are 11 known Ly-6 genes on murine chromosome 15 that code for different proteins. These family members, excluding secreted Ly6/Plaur domain containing 1 coded by the gene, are attached to the cell surface by a GPI anchor near the C terminus. The structure of these proteins may play a role in transmembrane interactions, and downstream signaling cascades (1,2). Ly-6 proteins have been widely used as differentiation markers on hematopoietic cells. The ability to isolate and express specific Ly-6 antibodies through hybridoma technology has allowed researchers to identify unique proteins (1). These proteins are expressed on subsets of immune cells at different stages of development, such as T cells, B cells, monocytes, granulocytes, and macrophages (1-5). and are two distinct genes located adjacent to each other within the Ly-6 gene complex. They share >95% similarity in their genomic and protein sequences, and with a nearly identical structure, expression profile, and reactivity to antibodies, they are collectively known as Ly-6C. A propeptide is removed to form the mature Ly-6C protein, a 10 kDa GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is most frequently used to distinguish inflammatory states of monocyte populations in mice (6). Alternate Names AA682074; AA959465; Ly-6; Ly-6C; Ly-6C.2; Ly-6C1; Ly-6C2; Ly6; Ly6c; Ly6c1; Ly6c2; lymphocyte antigen 6 complex, locus C; lymphocyte antigen 6 complex, locus C1; lymphocyte antigen 6 complex, locus C2; lymphocyte antigen 6C precursor (Ly-6C); Lymphocyte antigen 6C1; Lymphocyte antigen 6C2; Lymphocyte antigen Ly-6C; lymphocyte differentiation antigen Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 10 Source/Isotype: Rabbit IgG