CST, 37881S, GW182 (E4C7J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying GW182. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, GW182 (E4C7J) Rabbit Monoclonal Antibody (CST #37881) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity GW182 (E4C7J) Rabbit Monoclonal Antibody recognizes endogenous levels of total GW182 protein. This antibody detects both GW182 (Uniprot #Q8NDV7-2) and GW220/TNGW1 (Uniprot #Q8NDV7-1). Species Reactivity: Human, Mouse, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human GW182 protein. Background GW182/TNRC6 (GW) proteins are essential scaffolding proteins that play a critical role in microRNA (miRNA)-mediated gene silencing, a fundamental mechanism for post-transcriptional regulation of gene expression. GW proteins associate with members of the Argonaute (Ago) protein family to form the miRNA repressor complex (miRISC) and help target miRISC to specific mRNAs (1,2). GW proteins additionally interact with poly(A)-binding protein (PABP) and the CCR4-NOT deadenylase complex to carry out miRNA-mediated silencing and degradation of target mRNA (3). GW and Ago proteins are also known to associate in small cytoplasmic foci known as processing bodies (P-bodies) (4,5). The exact biological role of P-bodies has not yet been determined; these structures may play a role in mRNA degradation and storage, or are simply formed as a consequence of gene silencing (6,7). P-bodies that contain GW proteins are considered to be a distinct subtype referred to as GW/P-bodies, which sequester unique populations of non-translating mRNA (8,9). Three GW paralogs are expressed in vertebrates: GW182 (also referred to as TNRC6A), TNRC6B, and TNRC6C. These paralogs are believed to carry out partially redundant biological functions (10). GW182 is predominantly expressed as a 182 kDa protein but can also be expressed as a 220 kDa isoform (referred to as GW220/TNGW1) that plays a unique role in the formation of GW/P-bodies (8,11). Alternate Names CAG repeat protein 26; CAGH26; CTD-2540M10.1; DKFZp666E117; EDIE; EMSY interactor protein; FAME6; FLJ22043; Glycine-tryptophan protein of 182 kDa; GW1; GW182; GW182 autoantigen; KIAA1460; MGC75384; Protein GW1; TNR6A; TNRC6; TNRC6A; trinucleotide repeat containing 6A; trinucleotide repeat containing adaptor 6A; Trinucleotide repeat-containing gene 6A protein Specification REACTIVITY: H M Mk SENSITIVITY: Endogenous MW (kDa): 182, 220 Source/Isotype: Rabbit IgG