CST, 3790S, Phospho-HER4/ErbB4 (Tyr984)/EGFR (Tyr978) Antibody

Polyclonal Antibody for studying HER4/ErbB4 (Tyr984) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-HER4/ErbB4 (Tyr984)/EGFR (Tyr978) Antibody (CST #3790) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-HER4/ErbB4 (Tyr984)/EGFR (Tyr978) Antibody detects endogenous levels of HER4/ErbB4 protein only when phosphorylated on Tyr984 and EGFR only when phosphorylated on Tyr978. This antibody may cross-react with other tyrosine-phosphorylated proteins. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr984 of human HER4/ErbB4. Antibodies are purified by protein A and peptide affinity chromatography. Background Research studies have implicated the HER/ErbB receptor tyrosine kinase family in normal development, cardiac function and cancer (1,2). HER4/ErbB4, like other family members, has four ectodomains, a single transmembrane domain and a cytoplasmic tail containing the active tyrosine kinase domain (3). By binding to neuregulins and/or EGF family ligands, ErbB4 forms either a homodimer or heterodimer with other ErbB family members, which results in receptor activation and signaling (3). ErbB4 is ubiquitously expressed with the highest expression occurring in brain and heart. The expression of ErbB4 in breast cancer, pediatric brain cancer and other types of carcinomas has been reported in research studies suggesting that ErbB4 expression is involved in both normal tissue development and carcinogenesis (3). Alternate Names 4ICD; ALS19; avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4; E4ICD; erb-b2 receptor tyrosine kinase 4; ERBB4; ERBB4 intracellular domain; HER4; human epidermal growth factor receptor 4; MGC138404; p180erbB4; Proto-oncogene-like protein c-ErbB-4; Receptor tyrosine-protein kinase erbB-4; s80HER4; Tyrosine kinase-type cell surface receptor HER4; v-erb-a erythroblastic leukemia viral oncogene homolog 4; v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian); v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 4 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 180 SOURCE: Rabbit