CST, 37923S, LASS1 (F1D4P) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying LASS1. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, LASS1 (F1D4P) Rabbit Monoclonal Antibody (CST #37923) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity LASS1 (F1D4P) Rabbit Monoclonal Antibody recognizes endogenous levels of total LASS1 protein. This antibody does not cross-react with LASS2 or LASS6 and, based on homology, is not predicted to cross-react with other LASS family members. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp324 of human LASS1 protein. Background The mammalian ceramide synthases (CerS), also known as longevity assurance homologues (LASS), are a family of proteins that catalyze the formation of ceramide from acyl-CoA and sphingosine as part of the ceramide salvage pathway (1). Sphingosine, derived from the lysosomal breakdown of complex glycosphingolipids, can be delivered to the endoplasmic reticulum for LASS-dependent ceramide production (1). LASS family members exhibit preferences for different acyl-CoA fatty acid chain lengths (2-6) and have distinct tissue expression patterns, with notable enrichment of LASS1 in brain, LASS2 in liver and kidney, LASS3 in testis and skin, and LASS6 in intestine (6,7). Similarly, LASS proteins may function as tumor suppressors or promoters depending on tumor origin and metabolic context (8-11). Alternate Names Ceramide synthase 1; CerS1; Embryonic growth/differentiation factor 1; EPM8; GDF-1; GDF1; LAG1; LAG1 homolog, ceramide synthase 1; LAG1 longevity assurance homolog 1; LASS1; longevity assurance (LAG1, S. cerevisiae) homolog 1; Longevity assurance gene 1 protein homolog 1; MGC90349; Protein UOG-1; UOG1; upstream of GDF1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 29 Source/Isotype: Rabbit IgG