CST, 3793S, CIITA Antibody

Polyclonal Antibody for studying CIITA. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, CIITA Antibody (CST #3793) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CIITA Antibody detects transfected levels of total CIITA protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu149 of human CIITA. Antibodies are purified by peptide affinity chromatography. Background MHC class II (MHC-II) proteins play critical roles in cellular immune responses and their expression is mainly regulated by the non-DNA binding transcription factor CIITA (MHC class II transactivator) (1,2). CIITA expression is upregulated by IFN-γ and it in turn enchances MHC-II expression and represses collagen expression (3,4). CIITA has a limited number of transcriptional targets, most of which are involved in MHC-mediated antigen presentation (5). Mutations in the CIITA are associated with the hereditary immunodeficiency disease Bare Lymphocyte Syndrome (BLS) which is characterized by a nearly complete absence of MHC-II expression (also referred to as MHC-II deficiency) (6,7). Alternate Names C2TA; CIITA; CIITAIV; class II major histocompatibility complex transactivator; class II transactivator; class II, major histocompatibility complex, transactivator; MHC class II transactivator; MHC class II transactivator type I; MHC class II transactivator type III; MHC2TA; NLR family, acid domain containing; NLRA; nucleotide-binding oligomerization domain, leucine rich repeat and acid domain containing Specification REACTIVITY: H SENSITIVITY: Transfected Only MW (kDa): 140 SOURCE: Rabbit